Often asked: Which pump used in hplc?

Most HPLC pumps are reciprocating pumps. The solvent is drawn into a small chamber (with the solvent check valve open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor driven piston.

What is the pump in HPLC?

An HPLC pump is also called a “solvent delivery system.” The purpose of the pump is to maintain a constant flow of mobile phase through the HPLC system. … A standard HPLC pump can pump against back pressures up to 6,000 psi where a uHPLC pump typically exceeds 8,500 bar and can go as high as 20,000 psi or more.

Which of the pump is not used in HPLC?

Which of the following is not an advantage of Syringe type pumps used in High-pressure liquid chromatography? Explanation: The limitation of Syringe type pump is that it has the limited solvent capacity and is inconvenient when solvents are to be changed. 4.

Which pump is most commonly used high-pressure pump in HPLC?

Binary Pumps (two solvent gradient formation) are the most popular HPLC pumps on the market. Binary pumps are typically high-pressure mixing pumps that have gradient formation of two solvents.

Which column is used in HPLC?

The reversed-phase HPLC column is the most versatile and commonly used column type and can be used for a wide range of different types of analytes. Normal-phase HPLC columns have polar packing. The mobile phase is nonpolar and therefore usually an organic solvent such as hexane or methylene chloride.

How many types of pumps are used in HPLC?

Types of HPLC pump hplc-pump-types

Three common types of pumps are constant pressure pumps, syringe type pumps and reciprocating piston pumps. Figure 1. Constant pressure pump. Constant Pressure Pumps: As the name suggests, they provide constant flow rate in the column thanks to a constant pressure.

What is a binary pump?

the binary pump is the high mixing efficiency, which is necessary if TFA is used as a. modifier. At high flow rates and fast gradients, both pumps show excellent retention. time precision. The quaternary pump has the advantage that ternary and quaternary.

What is the detector in HPLC?

HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase.

Which detector is not used in GC?

Explanation: UV visible spectrometric detector is not used in gas chromatography. It is used in liquid chromatography.

Which pump is used for gradient elution?

Gradient elution with one pump

Two LAMBDA peristaltic pumps are used to mix the eluents for gradient elution.

What are peristaltic pumps used for?

Peristaltic pumps are a type of positive displacement pump used for pumping a variety of fluids. The fluid is contained within a flexible hose or tube fitted inside the pump casing.

What is isocratic pump in HPLC?

The 1260 Infinity II Isocratic Pump provides isocratic solvent management for HPLC, UHPLC, and GPC/SEC applications. This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. … This pump is ideal for GPC/SEC applications where run-to-run and day-to-day precision of retention times is crucial.

What is an isocratic pump?

The VERITY® 3011 Isocratic Pump is an innovative liquid delivery solution for chemical reaction monitoring in petroleum applications and for gel permeation chromatography (GPC) in environmental and food and beverage testing applications.

Why C18 column is used in HPLC?

C18 is considered better for separating compounds such as long-chain fatty acids as compared to relatively small organic compounds. It is relatively cheap as it is produced in large amounts by most manufacturers.

Why silica is used in HPLC column?

Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases. … The material filled in the HPLC columns is known as a stationary phase.

Is C18 polar or nonpolar?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.

How many types of pumps are present *?

There are three basic types of pumps: positive-displacement, centrifugal and axial-flow pumps.

What is a pneumatic pump?

What is a pneumatic pump? … Pneumatic pumps, in particular, use compressed air to create force that is used to move fluids through a piping system. Their system of operation is very similar to that of hydraulic pumps. Essentially, pneumatic pumps use air in the same way that hydraulic pumps use fluids.

What is a type of reciprocating pump?

There are three types of mechanism defined reciprocating pumps: piston, plunger and diaphragm or bellows. Valves at each end of the volume alternately close and open to isolate the higher pressure volume from the suction on the compression stroke and the higher pressure discharge from the volume on the return stroke.

What is quaternary pump?

The 1260 Infinity II Quaternary Pump is a gradient pump that uses low-pressure mixing for UHPLC gradient formation and solvent blending from up to four solvents. This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min.

What is high pressure gradient?

High pressure gradient systems are composed of two pumps. These systems are limited to two solvents which are pumped by the separate pumps. The mixer is located on the high pressure side of the pump. The mobile phase mixture is controlled by the relative flow rate of the two pumps.

What is quaternary gradient?

The 2535 Quaternary Gradient Module is a four solvent, low pressure mixing gradient pump (up to 50 mL/min) that serves as a solvent delivery device for purification systems. Dual programmable flow paths enable the system volume to change when the column size is changed.

Which lamp is used in HPLC?

Deuterium lamps emit an almost continuous spectrum of light ranging from the main UV wavelengths of 160 – 400 nm to the visible spectral range (800 nm). This makes them the ideal light source for high precision absorption measurements, e.g. in HPLC.

Why UV detector is used in HPLC?

HPLC UV/Visible detectors are used with high performance liquid chromatography to detect and identify analytes in the sample. The analyte can be identified by measuring the sample’s absorption of light at different wavelengths. … Therefore, it is important to choose an appropriate wavelength based on the type of analyte.

Can HPLC detect methanol?

A high performance liquid chromatography (HPLC) method for the determination of trace methanol in high water content sample has been established. … The result showed that methanol contents in all samples were below regulation standard (&lt, 0.1%, v/v).

What are the 4 types of chromatography?

There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography.

Which gas is used in gas chromatography?

Carrier Gas Types. Carrier gas is an inert gas used to carry samples. Helium (He), nitrogen (N2), hydrogen (H2), and argon (Ar) are often used. Helium and nitrogen are most commonly used and the use of helium is desirable when using a capillary column.

What is the difference between MS and FID detectors in GC analysis?

The FID measures the currant in a flame which is based on the ions of the burning organic compounds. The chromatogram of the MS (I think you mean the TIC) is based on the intensity of fragments produced by the ionization.

What is low pressure pump?

Low-pressure pumps are centrifugal pumps with heads up to 80 m (e.g. chemical pumps). Alternatives for other heads are the medium-pressure, high-pressure and super-pressure pumps.

What is HPLC gradient?

Gradient elution in HPLC refers to the technique of altering the composition of the mobile phase during the course of the chromatographic run.

What is a constant pressure pump?

Constant Pressure Pumps provide an even water pressure throughout your home or business. Constant pressure controllers use state-of-the-art technology to provide constant water pressure through variable speed control of submersible water well pumps.

What is Rotary Lobe pump?

Rotary lobe pumps are positive-displacement type pumps that use two or more lobes rotating around parallel shafts in the pump’s body to move liquids. They are widely used in the hygienic processing industries, including food &amp, beverage processing and biopharmaceutical manufacturing.

What is roller pump?

A peristaltic pump, also commonly known as a roller pump, is a type of positive displacement pump used for pumping a variety of fluids. … Typically, there will be two or more rollers compressing the tube, trapping a body of fluid between them. The body of fluid is transported through the tube, toward the pump outlet.

Which is positive displacement pump?

A Positive Displacement pump (PD pump) is a mechanical device which displaces a known quantity of liquid for every revolution or cycle that the pump completes. The flow rate through a positive displacement pump is directly proportional to its speed and number of cycles over a given time.

What is wet prime in HPLC?

Wet prime pumps (often called ‘self-primers‘) require the casing to be filled with water prior to the first start-up only. After that, the liquid will always remain in the pump volute, so the suction hose can run dry without damaging the pump or seals.

Which detector is not used in HPLC?

A UV detector cannot be used with solvent which has UV absorbance. Sometimes the organic solvent used for GPC analysis absorbs UV, and thus UV detector cannot be used. It provides a direct relationship between the intensity and analyte concentration.

What is the difference between binary and quaternary pump in HPLC?

The quaternary pump adds slightly more organic phase to the composition mixture resulting in shorter retention times. The results are presented in Figure 2. The binary pump shows slightly better results for the first two peaks, but over- all the results are comparable.

What is the difference between isocratic and gradient HPLC?

Isocratic means that the mixture of your mobile phase is consistent over the complete testing time. Using a gradient implies that the compounding of the eluent mixture is changed during measurement and so influences the retention of analytes. The separation can be either accelerated or decelerated.

What is gradient elution and isocratic elution?

The key difference between isocratic and gradient elution is that isocratic elution refers to maintaining a constant concentration in the mobile phase, whereas gradient elution refers to maintaining a varying concentration in the mobile phase. The terms isocratic and gradient elution are used in chromatography.

What is ODS and BDS column?

ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.

What is polar column?

Polar columns most effectively separate polar compounds. Non-polar compounds are generally composed only of carbon and hydrogen atoms and contain carbon-carbon single bonds. … Alcohols, amines, carboxylic acids, diols, esters, ethers, ketones, and thiols are typical polar compounds analyzed by capillary GC.

What is C8 column in HPLC?

C8 column is one of the columns used in HPLC in the reverse-phase. Generally, it contains octyl carbon chains, which are shorter. Therefore, its hydrophobicity is comparatively low. Also, this makes low retention time of nonpolar molecules in the column.

What is cyano column?

Answer: Cyano columns are usually prepared by bonding a cyanopropyl silane to the silica. This results in a column that has some polar functionality due to the CN group and some nonpolar functionality due to the propyl group.

Is silica polar or nonpolar?

Silica gel is a polar adsorbent. This allows it to preferentially adsorb other polar materials. When it comes to polarity, materials interact more with like materials. This principle is particularly important to many laboratories, which use silica gel as the stationary phase for column chromatography separations.

What is the formula of silica?

Summary: Silica is another name for the chemical compound composed of silicon and oxygen with the chemical formula SiO2, or silicon dioxide. There are many forms of silica.

What is ODS C18?

Reversed-Phase C18 Columns (ODS) 78. 06. Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety.

What is L1 column in HPLC?

According to the United States Pharmacopeia (USP), L1 column packing is defined as “octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter.” Some USP methods specifying an L1 column use intermediate pH, and many of these methods were done on a 10 m, 3.9 x 300 mm column.

What is C4 column?

YMC-Pack C4 (Butyl) columns are less hydrophobic than C8 or C18 packings, and generally utilize more aqueous eluents than either of these reversed phase column types. When compared to C8 and C18 columns using the same eluent, C4 columns show significantly shorter retention for non-polar compounds.